MOH Liberia E-Learning Platform

Vaccine reconstitution and dosage

This course is to enhance participant key knoweldge on good leadership sytle.

Syllabus

Module1

Introduction to Vaccine Reconstitution

1.1 What is Reconstitution?

Reconstitution is the process of mixing a lyophilized (freeze-dried) vaccine with a specific diluent to make it ready for administration.

1.2 Why Reconstitution Is Necessary

Maintains vaccine stability
Extends shelf-life
Ensures correct potency for immunization

1.3 Types of Vaccines That Require Reconstitution

Common examples:

BCG
Measles
MR / MMR
Yellow Fever
Men A conjugate (some presentations)

Module 2

Essential Principles of Safe Reconstitution

2.1 Use Only the Manufacturer-Provided Diluent

Each vaccine must use its matched diluent
Never mix different brands or interchange diluents

2.2 Check Before Reconstituting

Check:

Expiry date of vaccine and diluent
Vial integrity (cracks, leakage)
Vaccine vial monitor (VVM) status
Storage conditions
Correct dose and diluent volume

Module 3

Step-by-Step Reconstitution Procedure

3.1 Required Supplies

Reconstitution syringe (sterile)
Auto-disable (AD) syringes for administration
Diluent vial
Lyophilized vaccine vial
Alcohol swabs
Safety box
Hand hygiene supplies

3.2 Reconstitution Steps

Wash hands or use hand sanitizer
Verify the vaccine + diluent match
Inspect each vial
Clean vial stoppers with alcohol
Draw up diluent using a sterile reconstitution syringe
Inject diluent into the vaccine vial
Swirl gently (never shake vigorously unless specified)
Ensure the powder fully dissolves
Label the vial with time of reconstitution
Follow time limits for use (usually 6 hours after reconstitution, depending on vaccine)

Facilitator

Julien Boussou N'GBOSSO

IT engineer